FastQCFastQC Report
Thu 26 May 2016
ERR1356953_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356953_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615912
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28300.17513329933808278No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG8250.038.88630319
CGACGAG8400.037.3694224
ATCGTCA8400.036.95990810
TGGTCGT10800.036.74996651
GTCAGCG11650.035.2372643
GAGTCCT8950.035.078428
TAAATCG400.002598548834.49484324
GCTCGAT11250.034.34578764
GTCTACC11700.034.2180356
GTCGTCT11700.033.92304653
CTCGATA11300.033.5884165
GTCATCC9450.033.58228313
GCGCTGG12400.033.10289847
TCAAGAC3200.032.365973
TCGTCTA12350.032.1376254
TCGATAA11850.032.02945366
TGCGACG10000.031.39030822
GCTCGTC13400.030.62589839
CTGGTCG13650.029.83357450
TCAGCGC13950.029.67482444
Produced by FastQC (version 0.11.5)