FastQCFastQC Report
Thu 26 May 2016
ERR1356953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615912
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA8800.035.6988910
CGACGAG8950.035.4522724
GTAGCGC400.002598382534.4952963
CGGCGAT501.8652793E-434.4888932
CAATGCG9200.034.11506719
TGGTCGT10250.033.6664551
GAGTCCT9500.033.03672828
GTCATCC9800.032.3783313
GTCAGCG11050.031.835943
GCGCTGG11300.031.45429647
GATGCGG553.2637658E-431.35353728
GTCTACC11400.031.17838156
GCTCGAT10750.031.1317764
GTCGTCT11100.030.76036553
CTCGATA10950.030.5631565
TCGATAA11400.029.96293366
TGCGACG10600.029.93375422
GTACTAG12300.028.9382481
TCGTCTA12000.028.7540954
TCAGCGC13250.028.111744
Produced by FastQC (version 0.11.5)