ERR1356951_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1356951_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1584961
Sequences flagged as poor quality	0
Sequence length	75
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG	5167	0.32600171234497255	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	4997	0.3152758963785229	TruSeq Adapter, Index 10 (96% over 28bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC	3323	0.20965815562654222	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA	2573	0.16233837930397027	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA	2409	0.15199112154810118	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG	2354	0.14852100461777923	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT	2281	0.14391521305571559	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA	2123	0.13394651351042705	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG	2094	0.13211681549262097	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	1783	0.11249488157752778	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT	1745	0.1100973462438508	No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCACAAGCACGATCGGCTCCTAAT	1745	0.1100973462438508	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	1712	0.10801527608565763	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA	1680	0.10599629896256121	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT	1622	0.102336902926949	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTG	1621	0.10227380989185222	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG	1611	0.10164287954088459	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG	1608	0.10145360043559433	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTCTCG	20	0.0073689576	51.733273	41
TACCCCG	55	1.4721627E-7	43.901825	5
CTAGATA	40	5.0124396E-5	43.11106	19
GGAGTTC	245	0.0	40.823536	21
CCGTGTT	85	6.548362E-11	40.6174	9
CGACGAG	930	0.0	39.68072	24
GCTTGAC	45	9.9992234E-5	38.34514	56
CGGCGAT	45	1.0036457E-4	38.320946	32
GAGTCCT	945	0.0	38.320946	28
CAATGCG	955	0.0	38.280815	19
ATCGTCA	965	0.0	37.923603	10
ACCCTTG	295	0.0	37.448254	7
CCTTGTG	270	0.0	35.766212	27
TTCCTTG	270	0.0	35.766212	25
GTCAGCG	1205	0.0	34.631954	43
TGAACCG	100	3.8198777E-10	34.49429	5
CAGGACT	40	0.0026007504	34.48885	30
CACCGTA	40	0.0026007504	34.48885	29
GTCATCC	1075	0.0	34.009754	13
TGGTCGT	1220	0.0	33.65775	51

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content