FastQCFastQC Report
Thu 26 May 2016
ERR1356950_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356950_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842385
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG3600.033.53922322
CGACGAG4200.028.74790824
CAATGCG4950.025.09044619
CGCGATA2800.024.6430714
ACGTGCG2800.024.6397239
CGATAAT5350.023.8645667
GTATCAA36750.023.8632561
CTCGATA5300.023.43862265
GCGCGAT2950.023.38876213
GTACTAG6100.023.2063791
ATGCGAC5500.022.58017321
TCGTCTA5350.022.57826454
ATCGTCA5100.022.3225110
GCTCGAT5950.022.03260864
GCGATAT3450.022.0001915
TCGATAA5800.021.41805366
GGTATCA25200.021.2365761
CTAACGC3650.020.8105323
AATGCGA6050.020.52854520
ACGAGTC6050.020.525226
Produced by FastQC (version 0.11.5)