FastQCFastQC Report
Thu 26 May 2016
ERR1356950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842385
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT500.007730643327.60399664
ACGCGTA500.007731667727.60324965
CTCGATA5650.026.87041965
CGATAAT5550.026.72634367
TGCGACG4700.025.67986922
CGTCTAC6400.025.34167155
TGGTCGT6500.024.95247851
GCTCGAT6450.024.07325464
CGACGAG5050.023.90007624
CTGGTCG6850.023.67753450
TCGTCTA6750.023.51643254
GTACTAG6200.023.4023151
TCGATAA6600.023.00270766
GTCGTCT6900.022.98959753
CAATGCG5300.022.12206619
GTCTACC7550.021.94469856
GTATCAA39200.021.8558081
CGTCATT7600.021.32587626
AGGTCGT7950.021.25453222
ATGCGAC5900.021.04132721
Produced by FastQC (version 0.11.5)