FastQCFastQC Report
Thu 26 May 2016
ERR1356949_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356949_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774235
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20300.034.0107081
GTATCAA30850.024.0583041
TCAACGC36700.020.1293334
ATCAACG37150.019.8855063
CAACGCA37300.019.712995
TATCAAC38350.019.353292
AACGCAG40100.018.5869166
GTACATG56750.016.9106641
TACATGG57000.016.5942422
ACGCAGA44500.016.5898117
TTAATAC5250.015.7809683
ACATGGG58400.015.7235463
GTGTAGC7800.015.4901331
CGCAGAG47550.015.2354918
AGAGTAC44650.015.14336111
GTACCGA1605.7523197E-415.096496
GTTTACG1400.00411543714.7946581
CATGGGG44800.014.7176034
ATAGGAC2851.01148544E-714.5351033
CAGAGTA49900.013.61925510
Produced by FastQC (version 0.11.5)