FastQCFastQC Report
Thu 26 May 2016
ERR1356949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774235
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.027.8029651
GTATCAA29550.025.5945641
TCAACGC36100.020.2581544
ATCAACG36400.020.185963
TATCAAC37450.020.0805632
CAACGCA36500.020.0361465
AACGCAG39200.018.6713686
GTATTAG2651.1459633E-1018.2449761
GTGGTAT12600.018.0898321
GTCCTAC4050.017.9071061
CCGTCGA1200.001470222717.2622079
GTACATG59100.016.3033871
ACGCAGA45250.015.7934897
TACATGG60750.015.729132
ACATGGG60750.015.3884273
TGGTATC13900.015.3867922
CGATAAT2051.4436107E-515.146378567
CGCAGAG47250.015.0519148
GTACGGG1400.004104151414.8007711
CGATTGT1400.00412807814.78783447
Produced by FastQC (version 0.11.5)