FastQCFastQC Report
Thu 26 May 2016
ERR1356948_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356948_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803592
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG44670.24767242258781366No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC31640.17542770205234887No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA22650.12558272602672887No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG22400.12419660322290184No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA21990.12192336182462553No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA21940.12164613726386013No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20880.11576897657563352No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT20690.11471552324472498No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG20090.11138882851554009No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG9200.041.2484919
ATCGTCA9450.039.78998610
TGGTCGT10100.037.9260351
GAGTCCT10150.037.0468828
GCTCGAT11100.035.12423764
GTCATCC10950.034.02426513
GCTCGTC11650.033.4502339
GCGCTGG12000.033.05945647
GTCAGCG12450.031.31037143
TCAGCGC12700.031.2372844
GTCTACC12500.030.92030556
CGCCAGC12900.030.74956934
CTACACT12250.030.41352733
TGCGACG12500.030.35383622
TCATCCA12300.030.29157614
CGTCATT13100.030.01680626
GTCGTCT13000.029.73106453
GTACTAG13700.029.4836941
CTCGATA13500.029.13954465
GTCCTAC12950.029.03593630