FastQCFastQC Report
Thu 26 May 2016
ERR1356948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356948_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803592
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG44310.24567640575030272No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA35140.1948334213059273RNA PCR Primer, Index 39 (96% over 30bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC30340.16821986347244833No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG25680.14238253440911247No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA22950.12724607339132132No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA21370.11848577727113449No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA21270.11793132814960368No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG21030.11660065025792972No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG19660.1090046972929576No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGG200.007370321551.7311443
TCGCTGG707.2759576E-1249.3224512
TACCCCG405.006556E-543.1200455
CGACGAG8000.041.81600624
CAATGCG8250.040.54885519
GAGTCCT8350.040.0632428
ATCGTCA8500.039.80611810
TATTCGC753.9965016E-836.827439
TGGTCGT9600.033.78767451
GTCATCC10650.032.05962413
TGCGACG10600.031.88460422
GCTCGAT10150.030.93691664
GTATTGA450.00459843530.70061
ACGTTAG450.004631631530.6554918
CGAAGCT802.7706574E-630.17649828
GCGCTGG10850.029.89508447
ACCGTGC1052.3263055E-829.57951255
TAGCTAA703.8618084E-529.56065232
GTCTACC11000.029.49396956
GTACTAG12200.029.442381
Produced by FastQC (version 0.11.5)