FastQCFastQC Report
Thu 26 May 2016
ERR1356943_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356943_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828835
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA3100.032.279965
CGTACGA2150.032.08728460
TAGCGTA2300.031.4995457
CGATAAT3300.031.36918667
GGTATCA24350.030.3431261
GTATCAA36450.024.627561
CTAGCGT3000.024.15559456
GCTCGAT4350.023.79340664
TCTAACG1352.6418093E-723.0172942
CGTCATT5100.022.32014726
GCGTACG3150.021.89904859
TTACTAG3200.021.55982443
GTCTACC4850.021.3451556
CATTGCG5100.020.97142829
TGCGACG2501.8189894E-1220.69686522
TCGTCTA4900.020.42309654
ATCAACG43100.020.1868613
TACCTGG11650.020.1524812
CTAACGC1559.979103E-720.0473213
TAGGTCG5350.019.98762521
Produced by FastQC (version 0.11.5)