FastQCFastQC Report
Thu 26 May 2016
ERR1356943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828835
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25200.030.4371661
CGATAAT3000.029.90100767
CTCGATA3600.025.87728565
GTACTAG2900.025.019181
GTATCAA38150.024.2712441
CGCACTA2150.024.0791458
ATGCGAC2750.022.57516921
GCTCGAT4450.022.48513464
TGCGACG2850.020.5728922
ATTAGGT4400.020.3803619
TATCAAC45450.020.3405822
ATCAACG45250.020.2780173
GTATAGA2900.020.2536221
CAACGCA45350.020.157245
CGTTATT1206.404211E-520.11907444
TCAACGC45450.020.112894
CAATGCG3100.020.02636119
TAGTACT5200.019.901254
TCGTCTA4350.019.82933854
CTAGTAC5050.019.8092983
Produced by FastQC (version 0.11.5)