FastQCFastQC Report
Thu 26 May 2016
ERR1356941_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356941_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744308
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18250.030.2728331
GTATCAA27500.025.6149441
CGCGATA750.00199402122.99883314
TCAACGC31650.021.8198724
CAACGCA31600.021.7432565
ATCAACG32500.021.1429543
TATCAAC33900.020.7790832
CGCACTA2201.9099389E-1020.38591429
AACGCAG34650.019.9192686
GTCCTAC4250.018.6867961
GTACATG52350.018.5347021
TACATGG51950.017.7468662
TGCGCGA1402.0850764E-417.24813712
ACGCAGA40400.016.9919767
CGCAGAG41000.016.5750378
GTATAGG4900.016.2079351
ACATGGG54850.016.179033
TACCTGG11500.015.9138032
CATGGGG41150.015.8594434
GTACCGT2201.6167269E-615.6864236
Produced by FastQC (version 0.11.5)