FastQCFastQC Report
Thu 26 May 2016
ERR1356941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744308
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19150.029.7608621
GTATCAA26800.022.4256361
TCAACGC30650.019.694274
CAACGCA30800.019.4863665
ATCAACG30800.019.4863663
TATCAAC32250.018.6102312
GTACTAG2457.894414E-1018.3276841
AACGCAG33100.018.1515836
TACGTTG950.00785285518.15114433
CGACGAA1150.001113311417.99330738
CGATAAT1954.599151E-717.69130767
GTACATG51650.017.387351
TACATGG51150.016.9936712
CGTCGTT1653.83585E-516.73112346
ACATGGG50900.016.4672373
TATACCG1503.531809E-416.0967835
TACCTGG11650.015.69214252
TCGTATG7000.015.27288940
AATCTCG7500.015.17435736
ACGCAGA39150.015.1701517
Produced by FastQC (version 0.11.5)