FastQCFastQC Report
Thu 26 May 2016
ERR1356938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791238
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAA6100.027.71414866
TGGTCGT6300.026.8395851
CGATAAT6200.026.71067267
CTGGTCG6600.026.14025950
GCTCGAT6400.025.87451764
CTCGATA6550.025.28338465
TCGTCTA6600.025.09675254
CGACGAG5250.024.97026624
GTCGTCT6800.024.35861253
GTACTAG6900.023.0182191
TGCGACG5700.022.99828722
GCTCGTC7500.022.53139939
CGTCTAC7300.022.217555
GTCAGCG7700.021.50429243
CAATGCG6100.021.48960119
GATCGCA5150.021.43529710
ATCGTCA6200.021.14358510
TCAGCGC7850.021.0933844
GTGATCG5100.020.9690278
GTCTACC7950.020.83503756
Produced by FastQC (version 0.11.5)