FastQCFastQC Report
Thu 26 May 2016
ERR1356938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791238
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG4450.033.32588624
TCGATAA6250.032.0213466
TGGTCGT6400.031.8108851
CTCGATA6450.030.49345665
CAATGCG5000.029.66169519
TCGTCTA6800.029.43384454
TGCGACG5200.029.18250522
CGATAAT7000.029.07854867
GCTCGAT6800.028.92474764
CTGGTCG7350.028.63822750
ATCGTCA5600.027.74333610
GTACTAG6750.027.1206761
GTCAGCG7900.026.19373543
GCTCGTC8250.025.91856639
CGTCTAC7900.025.7722855
GTCTACC8000.025.45083456
GCGCTGG8750.025.23919947
GTCGTCT7950.025.16136253
CGTCATT8400.025.0451626
TCAGCGC8850.023.38197544
Produced by FastQC (version 0.11.5)