FastQCFastQC Report
Thu 26 May 2016
ERR1356935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813624
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG9450.038.32798824
CAATGCG9750.038.21532419
ATCGTCA9850.036.4243310
TGGTCGT13800.034.25939651
GCTCGAT13750.033.11720764
GTCAGCG14850.032.52430343
CGAGTGA1700.032.47322565
CTGGTCG14850.032.0658650
GTGCGTT1850.031.70790150
TCGATAA14650.031.3233666
GTCATCC11600.031.22581513
GAGTCCT11800.030.98891328
GTCTACC15300.030.90062956
ATCGCCG1800.030.65224339
CGATAAT15100.030.16138867
TCGTCTA15800.030.14117854
CGGCGAT1501.8189894E-1229.89665432
TCAGCGC16050.029.87763444
CCAATGC12850.029.80150818
CTCGATA15450.029.70143965
Produced by FastQC (version 0.11.5)