FastQCFastQC Report
Thu 26 May 2016
ERR1356935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813624
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG51550.2842375266317605No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32910.18145988363629947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG9750.039.61743524
CAATGCG10000.038.28211619
TGGTCGT11400.036.6232751
ATCGTCA10200.036.22294210
CTGGTCG12300.033.6629950
GCTCGAT12250.032.9544764
GAGTCCT11650.032.56414828
GTCTACC12800.031.5462956
TCAGCGC13450.031.28315244
GAGATCG2100.031.20378536
GTCAGCG13600.030.68452643
AGTCCTA12400.030.59454229
GCGCTGG13550.030.55754747
GTACGCA2150.030.51513111
ATCGCCG2050.030.2824939
TCGATAA13550.029.7936166
GTCATCC12350.029.6046413
CGGCGGA605.41048E-428.76173861
TACCCCG1202.6921043E-928.7450835
CCAATGC13450.028.7189618
Produced by FastQC (version 0.11.5)