FastQCFastQC Report
Thu 26 May 2016
ERR1356934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233979
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG3800.031.77323524
CAATGCG4000.031.05076819
TGCGACG3900.030.95853622
ATCGTCA4050.029.81434410
GTACTAG4800.029.486221
CTCGATA2950.026.90224365
TTATGCG1359.369614E-925.5707044
GTCCTAC4800.025.15381230
TCGATAA3200.024.80050566
TCGTCTA3100.024.49043354
ATTGCGT1301.843564E-723.8900496
CGATAAT3500.023.66060667
CTGGTCG3550.023.33019650
TGGTCGT3450.023.00616651
GCATCGT5550.022.3762078
TATCTCG2950.022.21818423
TAGGCAT6450.021.9432335
AGTCCTA5350.021.92399629
GCTCGAT3650.021.73938264
ACGAGTC5400.021.71835326
Produced by FastQC (version 0.11.5)