FastQCFastQC Report
Thu 26 May 2016
ERR1356934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233979
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG3050.037.31397219
CGACGAG3000.036.7848124
TGCGACG3000.036.7848122
CTCGATA3200.035.58362265
TCGATAA3550.032.07537566
GTACTAG4600.031.5534821
CGATAAT3650.031.19154267
TGGTCGT3800.030.8731951
TCGTCTA3700.030.77627454
ATCGTCA3750.030.388110
GCTCGAT3750.030.36469364
GTCGTCT4200.027.09595353
CTGGTCG4250.026.79237750
ATCGCGA809.58927E-525.86431733
ATGCGAC5000.024.82974421
GTCCTAC4400.024.29678330
AATGCGA5150.023.43692220
CGCACTA4000.023.299658
AGTCCTA4600.023.24040229
CGTCTAC4950.023.00448855
Produced by FastQC (version 0.11.5)