FastQCFastQC Report
Thu 26 May 2016
ERR1356933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434176
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG4500.029.89871422
CAATGCG4650.029.67510819
GCGCGAA500.007727951727.60555652
ATCGTCA4900.027.45704510
ACGCAAC658.667375E-426.5336235
CGACGAG5100.026.3812224
TAGGCAT7050.024.4781365
CTCGATA6550.024.23134865
TCGATAA6500.023.88692366
GCTCGAT6600.023.52253764
ATGCGAC6700.022.6557421
GTCCTAC5800.022.60326630
GTATCAA41150.022.3951531
TCGGTAT3750.022.07905227
CGTTAAC2057.2759576E-1121.8877981
CGATAAT7250.021.41586167
CGGTATA3900.021.23059728
GTTTCGC2650.020.8300232
GTATTAG2001.2732926E-920.7092251
ACGAGTC6900.020.49840426
Produced by FastQC (version 0.11.5)