FastQCFastQC Report
Thu 26 May 2016
ERR1356933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434176
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA7450.031.95790165
GCTCGAT7650.031.57344864
CGACGAG3500.031.53227424
TGGTCGT8350.030.58058451
GTCGTCT8550.030.25300453
TCGATAA7850.029.88991466
TCGTCTA8600.029.69057754
GTCTACC8750.029.18363256
GATCGCA4300.028.9052710
CGTCATT9100.028.80351826
GTTACGC854.422691E-628.41709547
GTGATCG4600.027.7707048
TCAGCGC8950.027.74487944
CGATAAT8650.027.52251267
GCTCGTC9150.027.51535639
GTCAGCG9250.026.84504543
GTTATGC906.918206E-626.8280743
ATAAGGT5300.026.6834473
CAATGCG4350.026.16636819
CATTGCG9550.026.00174329
Produced by FastQC (version 0.11.5)