FastQCFastQC Report
Thu 26 May 2016
ERR1356932_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356932_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462352
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG33330.2279205006728886No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC20830.14244176504699277No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA19390.1325946147028896No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA18620.1273291245883344No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA17690.12096950665776776No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG16870.115362101600709No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT16390.11207971815267459No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT15400.10530980229110365No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA14980.10243771677407355No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG14930.10209580183156997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCT7200.031.6263828
CAATGCG7200.031.62205319
GTCAGCG9750.031.13342943
TGGTCGT9350.030.6364451
CTAGACA703.8281534E-529.5980384
CGACGAG7800.029.18958524
ACCCTTG3250.028.6578877
GGGTAAA1103.6328856E-828.2526741
ATCGTCA8250.028.01556810
GCTCGAT11200.026.7993664
GCGCTGG11500.026.39302347
GTCGTCT10750.026.32553553
GCTCGTC11400.026.31748839
CTCGATA11500.026.10203265
TGCGACG9050.025.91934822
TCAGCGC12150.025.55142644
GTCCTAC8950.025.43896930
AATAGCA4800.025.1771625
GATCTCG1101.154076E-625.0885733
GTACCCA1801.2732926E-1124.9390871
Produced by FastQC (version 0.11.5)