ERR1356932_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1356932_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1462352
Sequences flagged as poor quality	0
Sequence length	75
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	6853	0.4686286201954112	RNA PCR Primer, Index 27 (96% over 30bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG	3384	0.23140803308642513	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC	2099	0.14353589286300425	No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA	1915	0.13095342297887239	RNA PCR Primer, Index 27 (96% over 30bp)
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA	1881	0.12862840136984802	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA	1861	0.1272607415998337	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG	1763	0.12055920872676347	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA	1711	0.11700329332472618	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT	1655	0.11317384596868606	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA	1493	0.10209580183156997	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG	1487	0.10168550390056566	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCGT	15	0.0023587418	68.97736	39
TGGACAA	30	6.3201046E-4	45.988056	14
TACCCCG	60	2.914894E-7	40.243675	5
CGACGAG	685	0.0	35.243908	24
CAATGCG	690	0.0	34.98972	19
GTTTAGA	50	1.8362136E-4	34.58091	1
GAACTTA	50	1.8518063E-4	34.53119	11
TGAACTT	50	1.8518063E-4	34.53119	10
CGGCGCT	70	9.670057E-7	34.531185	10
GCGTGCA	40	0.0026007458	34.48868	29
GAGTCCT	700	0.0	33.99599	28
ACAGACA	105	6.4574124E-10	32.84749	17
ACCCTTG	305	0.0	32.83181	7
GTCAGCG	935	0.0	31.72221	43
ATCGTCA	755	0.0	31.5583	10
GGGTAAA	110	1.033186E-9	31.437191	1
GAGCGGT	45	0.0046227844	30.667091	69
CGCTACG	45	0.0046297503	30.657656	17
ACCATGG	45	0.0046305247	30.656605	35
GATTCAA	45	0.0046305247	30.656605	21