FastQCFastQC Report
Thu 26 May 2016
ERR1356931_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356931_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648002
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAT8500.033.68922864
CGACGAG5650.033.57996424
CGATAAT8350.033.4721267
TCGATAA8700.032.52215266
CAATGCG6000.032.19801319
TGCGACG5900.032.15610522
TCGTCTA9050.032.0327754
CTCGATA8900.031.79131365
GTCGTCT9500.030.51542753
ATCGTCA6500.029.18962310
TGGTCGT9850.028.73038351
CGTCATT10550.027.13803526
GTCTACC10700.027.09313856
GTACTAG8100.026.8496151
CTGGTCG10450.026.748150
GCTCGTC11000.026.64935739
AATGCGA7450.026.39434820
CGTCTAC10900.026.27939655
GTCGTCA11150.025.67847424
ATGCGAC7850.025.48655521
Produced by FastQC (version 0.11.5)