FastQCFastQC Report
Thu 26 May 2016
ERR1356931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648002
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTA8700.029.74565954
CGATAAT8400.029.57119667
TGGTCGT8900.029.46670351
TCGATAA8700.029.34815866
CTCGATA8800.029.01465865
CGACGAG5000.028.9692224
CAATGCG5250.028.24663219
GCTCGAT9200.027.7531564
CTGGTCG9650.027.17654450
GTCGTCT9550.026.7230453
GCTCGTC9750.026.5285939
GTCTACC10000.025.88186556
CGTCTAC10000.025.87872355
TGCGACG5700.025.41159622
TCAGCGC10750.024.70243544
GTCAGCG10650.024.61056143
ATCGTCA6500.024.43818510
TCCGATC851.4452352E-424.34905252
GCGCTGG10650.024.30074547
CATTGCG11150.023.19764729
Produced by FastQC (version 0.11.5)