FastQCFastQC Report
Thu 26 May 2016
ERR1356930_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356930_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357786
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG35030.25799352769876843No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT18240.1343363387161158No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA18210.13411539079059587No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA18110.13337889770552944No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA18050.1329370018544896No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC17820.13124306775883682No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14940.1100320669089238No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCA14870.10951652174937729No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT14690.10819083419625773No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG14250.10495026462196547No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA14060.10355092776033925No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA13600.1001630595690337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG6900.036.99523519
ATCGTCA7450.035.190110
GTACCCA1800.034.5388371
CTCGTGA400.002597669634.4968329
TGGTCGT9350.034.328851
GTCATCC7900.032.74894713
GAGTCCT7500.032.19822728
GTCCTAC7800.031.84205430
AGTCCTA7700.031.80876429
CTGGTCG10350.031.01086650
GTCTACC10150.030.94301856
GTCAGCG10400.030.84471743
CGACGAG7950.030.80622124
CTGTTCA450.004627561730.660469
CGCTTTA802.7657006E-630.182526
GGTCGCC1650.029.26680442
TGCGACG8700.028.94241522
GCGCTGG11100.028.58880847
CTACACT8100.028.53336333
GTCGTCT11500.028.51104453
Produced by FastQC (version 0.11.5)