FastQCFastQC Report
Thu 26 May 2016
ERR1356930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357786
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA75500.5560522792251503RNA PCR Primer, Index 17 (96% over 28bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG36600.2695564691343113No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA21890.1612183363210403RNA PCR Primer, Index 17 (96% over 28bp)
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA19220.14155397094976677No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA18600.13698771382235492No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC18460.13595662350326193No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA17650.1299910295142239No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT17490.12881264057811762No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG15920.11724969914257476No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCA14890.1096638203663906No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14720.10841178212177766No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG14480.10664419871761824No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA14130.10406647291988574No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA14040.10340362914332599No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT13910.10244618813273962No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACAC208.52958E-568.9757419
CCATCGG200.007354548751.75848864
AGGGTGC200.007366341551.7375213
CAAATCG200.0073695651.731818
CAATGCG6550.035.27766419
CGACGAG6600.035.0104124
GGGTAAA1550.033.448031
GTACCCA958.643838E-932.743861
GAGTCCT7100.032.05914328
AGTCCTA7300.031.18080929
TTTCGGA450.00460514130.69091610
GGTCGTG450.00460763330.6875237
ATCGTCA7450.030.12447210
TTAGCGT703.8607774E-529.56102817
TGGTCGT7950.029.5153651
GTCATCC7850.029.43875713
GTGTTAA953.153782E-729.1056521
TGCGACG8250.028.00832722
GTCTACC8350.027.69125456
TGGCTTA500.007734779327.60045469
Produced by FastQC (version 0.11.5)