FastQCFastQC Report
Thu 26 May 2016
ERR1356929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687978
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG38730.22944611837358073No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC23540.13945679386816653No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA21770.1289708752128286No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA21060.12476465925503769No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG20940.12405374951569276No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA19070.11297540607756736No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT17900.10604403611895416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA8400.037.37302410
CAATGCG8350.036.7694219
GAGTCCT8650.034.6986228
CGACGAG9400.032.66123224
GACTCAC1001.4460966E-831.0603353
GTCATCC10250.030.96329913
AATGCGG450.00462449230.66504927
GTCTATA1600.030.2065031
TCATCCA10900.029.43509714
TCAAGAC1650.029.2911533
CGACACT1202.6884663E-928.74933214
GCATATA2050.028.6277661
CATCGGT854.4346507E-628.40773825
GTCCTAC10850.027.9784330
CTACACT10750.027.917833
TGCGACG11150.027.84441222
ATACATG1503.45608E-1127.6165525
CGAGGTT500.007748004527.59117936
ACCCTTG3700.027.0383117
CCAATGC11450.026.8143818