FastQCFastQC Report
Thu 26 May 2016
ERR1356929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687978
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA61650.365229878588465TruSeq Adapter, Index 11 (96% over 28bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG40080.23744385294121131No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC25040.14834316560997834No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA22780.13495436551898188No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA22400.13270315134438956No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG21910.12980026990873103No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA18040.10687343081485659No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG17210.10195630511772072No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG17000.10071221307386707No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT150.002342530369.09791
GTGCAAC208.531828E-568.9730435
CGGTCTC200.007346863451.77272463
TAGACCG200.0073692851.732854
CCAGTGA352.2785985E-549.26646421
CGCTATA350.001348230439.42718569
TGCCGAA459.9813165E-538.3569961
GGGTAAA1500.036.852211
GTCTAGA501.8463391E-434.548951
GCCGAAT501.8550498E-434.5212962
CAGGGTC400.002596749234.49980567
CGGTGTT709.760588E-734.49061214
ATGCGAA501.8657422E-434.48754517
AACGCAT501.866067E-434.48652333
CGTATGG400.002601658734.4865222
CTAAGTT400.002601658734.4865232
ATCGTCA7550.033.38315210
TGGTCGT10650.032.72879451
GTCAGCG11050.032.45790543
CGACGAG7800.032.2758524
Produced by FastQC (version 0.11.5)