FastQCFastQC Report
Thu 26 May 2016
ERR1356926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762399
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAG127520.7235591940304097No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCG101860.5779621981174524No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG57950.3288131688681167No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGC55380.31423077294074725No Hit
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTG51860.29425799719586765No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGT46880.2660010587840778No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTC43560.2471630998428846No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC42780.2427373143085079No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT41960.23808456541339393No Hit
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGT41700.2366093035686017No Hit
CTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTAC33350.18923070201469702No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA32800.18610995580455958No Hit
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTA32650.1852588432017948No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG31210.1770881622152532No Hit
ACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGG30140.17101689231553127No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA28970.16437821401396618No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT27850.15802323991332268No Hit
ACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC26850.152349155894891No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA26260.1490014463240163No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA25530.14485936499056115No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT24860.14105772869821193No Hit
GGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAG24230.13748305576659997No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG23850.13532690383959592No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG22480.1275534087343445No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTG22470.12749666789416017No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT21280.12074450791222646No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA21190.1202338403505676No Hit
GCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACG19970.11331145784808094No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCA19780.11223338188457892No Hit
CATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT19570.11104182424070827No Hit
TACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCA19220.10905589483425718No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT18950.10752389214928061No Hit
GTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT18850.10695648374743744No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA18700.10610537114467268No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGATC17960.10190654897103324No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG17920.10167958561029596No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG17750.10071499132716258No Hit
GTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG17630.10003410124495077No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC306.302947E-446.0139553
TTGAGTG350.001348825939.423747
GTCTACC14000.036.2349656
TGGTCGT14300.036.19978751
GTCAGCG14800.035.19400843
GAGTCCT10050.035.01361528
CAATGCG10350.034.66438719
CTCCGTC400.002598270534.495777
CTGGTCG15050.034.3938650
GCGCTGG15500.034.27127547
ATCGTCA10550.034.00627510
AGTCCTA10250.033.9938529
TCAAGAC2050.033.6821373
TCAGCGC16200.032.79134844
GTCGTCT15800.031.67153
GTCATCC11350.031.60935813
ACCAGCT15950.031.35801160
TCGCTGC1001.4470061E-831.05853756
TCGTCTA16250.030.79395554
ATTCAAG2250.030.6881711
Produced by FastQC (version 0.11.5)