FastQCFastQC Report
Thu 26 May 2016
ERR1356923_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356923_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927556
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG3500.029.56909424
GCGACGT1254.1673047E-927.59280835
CAATGCG4200.027.10519
TGCGACG4100.026.92400222
GCTCGAT5250.026.28568664
CTCGATA5350.025.15146465
ATCGTCA4300.024.87206610
TGGTCGT5250.024.32057251
TCGTCTA5450.024.06126654
GTACTAG6050.023.9615821
TTACGTG6550.022.65059569
GTCGTCT5800.022.60929353
TGATCGC4150.022.4451759
TCGATAA6000.022.42672266
CGATAAT6200.021.7032867
GCTCGTC6750.021.45995939
CGACGTT1455.308775E-721.4087736
GTGATCG4400.021.1698828
ATGCGAC5550.020.51136221
CGATATA1356.8304107E-620.44336162
Produced by FastQC (version 0.11.5)