FastQCFastQC Report
Thu 26 May 2016
ERR1356923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927556
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG3500.027.59124424
TCTCGCG658.675291E-426.5300432
CAATGCG3750.025.75182719
CTCGCGC700.001336643124.63503833
GCTCGAT5300.024.0872664
TGGTCGT5250.023.66191351
GCTCGTC6100.022.61577239
TCGATAA5850.022.41244966
CTCGATA5850.021.82264765
TGATCGC3650.021.7554049
TCGTCTA5950.021.4558854
ATCGTCA4450.020.94767810
TATGCCG20450.020.91267843
TAGTCGG1657.950075E-820.90245640
CGATAAT6200.020.58965567
CTCGTAT21500.020.37260439
TGCGACG4800.020.11861422
ATCGGTC1206.405566E-520.11861426
CGTACGA3800.019.98030560
ACCGTCG3850.019.7080323
Produced by FastQC (version 0.11.5)