FastQCFastQC Report
Thu 26 May 2016
ERR1356921_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356921_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884456
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA360031.9105248411212572No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG145250.7707794716353155No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT25140.1334072008049007No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTA500.007742810527.59513526
CGTATAC658.619799E-426.5591743
ACGTGTA5450.022.15735269
AGCCGAT800.002902185321.55984523
ATACTCT19150.021.2728145
CTTACGT14850.021.14273869
GCTTATA19200.021.0398451
TACGTGT7600.020.88288969
TATACTC19700.020.68114
GCTTATG11350.019.1647241
AATACGT3150.018.61675524
CGTAGGC3001.8189894E-1218.39822428
GAATACG3200.018.32586923
TTATACT22300.018.2698523
TCAATCG950.007847832518.15324838
CGAATTA1150.001112291517.99587234
GTCCTAC8100.017.902851
TATGTAC12000.017.8389114
ATACGTA3103.6379788E-1217.80331425
TACGTAG3103.6379788E-1217.80331426
Produced by FastQC (version 0.11.5)