FastQCFastQC Report
Thu 26 May 2016
ERR1356921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884456
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA63840.3387715075332085No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG200.007370253651.73135414
TAATACG605.414727E-428.75795448
CCGGTTA700.001330571624.65425758
GCTTATG9450.024.14551
GCTTATA17650.023.7008931
TATACTC18850.022.506814
CTTATGT9700.022.4021152
CGATTAG1959.385985E-1021.22143218
AACGCTC1304.943382E-621.22142839
TATGTAC10900.020.8851534
ATACTCT19750.020.6079655
TATGCCG33500.020.48501443
AGTACTC6400.020.479715
TGCCGTC33650.020.30576545
ATGCCGT33650.020.2912244
TTATGTA11100.020.1981033
GCCGTCT33900.020.15601746
CTCGTAT33900.020.14157939
ATCGATA1206.41152E-520.11614623
CCGTCTT34000.020.09673547
Produced by FastQC (version 0.11.5)