FastQCFastQC Report
Thu 26 May 2016
ERR1356920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053135
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG1501.036824E-925.3122541
GTATCAA24600.024.1336331
GGTATCA16800.022.8056831
TCGGTAT953.069139E-421.78552625
TACGTGT3750.020.24499169
TCAACGC28900.019.8262064
ATCAACG28600.019.7927993
TGCGACG1751.475255E-719.71071622
CAACGCA28950.019.67185
TATCAAC29200.019.5043052
ATAGGAC1601.3552926E-619.4156483
GTATTAG1258.707684E-519.3293571
ATTATAC1258.707684E-519.3293573
AACGCAG30500.018.5545126
GTACATG41600.018.4199751
CGTGTAG1702.4261553E-618.26920969
CGCACTA1702.4394085E-618.25879758
TTATACT2651.1459633E-1018.2352434
GTGTACG950.007812184318.1666871
GCACTAA950.007812184318.1666871
Produced by FastQC (version 0.11.5)