FastQCFastQC Report
Thu 26 May 2016
ERR1356920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053135
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG400.002600173234.4893443
CTCGATA1052.3268512E-829.57633465
TGCGACG906.921995E-626.82504522
GTATCAA24800.025.6424351
GGTATCA16300.022.687641
TCGTCTA1756.19184E-921.68828454
CAACGCA28900.021.6016165
CCGTCGA800.00289010121.5742769
CGATAAT1455.286074E-721.41632867
ATCAACG29500.021.162263
TATCAAC30000.021.1544672
TCAACGC29950.020.959464
CCGATAG850.00412620120.288813
AACGCAG31250.019.9923636
TGTGCGT1056.000421E-419.7250528
GTATTAT1258.6213055E-519.3544641
GTACTAG2002.8214345E-819.008851
TCGATCG1108.2532235E-418.81236841
TCGGTAT1108.2532235E-418.81236825
CGTACAC1301.1832769E-418.5720653
Produced by FastQC (version 0.11.5)