FastQCFastQC Report
Thu 26 May 2016
ERR1356919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314657
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG501.8623602E-434.49748614
CGATAAT4000.025.87705467
GCGTAAA700.001329490324.6570171
TCGATAA4400.023.52459766
TCGTCTA4200.023.00883354
GCTCGTC4650.022.99307439
GTCGTCT4300.022.47374353
CTCGATA4650.022.25983265
GTATAAG4900.021.8390711
GCTCGAT4750.021.78954564
CGTCATT4950.021.60037626
TGCGACG2750.021.32409722
ATAGGAC2451.8189894E-1221.1345863
CGTCTAC4950.020.91712255
GTATCAA27850.020.69951
CGACGAG2850.020.57588224
AATATCG850.004122228420.2926430
TCAGCGC5300.020.17854344
GATCGCA3450.019.99854710
CAATGCG2800.019.7128519