FastQCFastQC Report
Thu 26 May 2016
ERR1356919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314657
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA16020.12185687977928844RNA PCR Primer, Index 27 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG2650.033.83827224
CGATAAT3250.029.72491367
TGCGACG3100.028.92626622
CAATGCG3350.026.76860619
GTACTAG4700.026.459381
CTCGATA3650.025.52503865
TCGTCTA3700.025.18010754
TCGATAA3900.024.77358666
ATCGTCA3900.023.8997310
AATGCGA4600.023.24259420
GCTCGAT4150.022.44973464
CGCACTA3400.022.33170758
ATGCGAC4300.021.65588821
CCCGTAT1154.5983907E-521.00289549
GATCGCA3950.020.9752910
GTCGTCT4450.020.92591553
GACGAGT4450.020.92591525
GTGATCG3800.020.8947948
GTATAAG5400.020.4706291
GCTCGTC5100.020.28765139
Produced by FastQC (version 0.11.5)