FastQCFastQC Report
Thu 26 May 2016
ERR1356918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797423
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG44050.24507308518918475No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA27330.15205101970988463No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC24930.1386985701195545No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG24210.13469283524245546No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA23400.130186383505719No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA20550.11433034961720195No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTG19370.10776539523528965No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG19180.1067083263093885No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG18900.10515054052384998No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG18800.10459418845758624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAGAC1450.042.8553053
ACGTATC400.002597703434.4973442
TCTCGGG501.8651172E-434.48966235
CAATGCG7050.033.76241719
ATCGTCA7100.033.03878810
GTCTGCA751.6712947E-632.1966211
GAGTCCT7200.032.10258528
TGGTCGT11350.031.92805951
GCATATA1305.456968E-1231.8667661
TACACAG553.2459444E-431.3830625
TATCACG553.26295E-431.3551134
CGACGAG7500.031.27584624
ATCGCTA450.004625749330.6634546
ACCCTTG3550.030.122767
GCTCGAT11900.029.86070664
GCGCTGG12550.028.85990747
GATATAC1202.6902853E-928.74618363
CACTTTG1202.6902853E-928.74618317
CGTTAAC3550.028.201341
GTCAGCG12800.028.0275343