FastQCFastQC Report
Thu 26 May 2016
ERR1356918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797423
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA59860.3330323468654846RNA PCR Primer, Index 26 (100% over 29bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG45600.25369654221627297No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA27730.15427642797493968No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC25800.14353883309604917No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG25750.1432606570629173No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA23040.1281835160671695No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA20540.11427471441057559No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20190.11232748217865243No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTG20180.11227184697202607No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG18670.10387093077144334No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCT18410.10242441539915757No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACAT18340.10203496895277295No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG18200.10125607606000367No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTGT200.00735332251.76135646
TTACGTA306.287445E-446.0370036
ACCATGC306.3090475E-446.0049764
TACCCCG651.14087015E-842.4531335
CGGCGAT1250.041.38489532
GCATATA1350.040.948011
GTACCGT350.001342702239.4602936
ATCATCC350.001346198739.4394256
TCAAGAC2500.038.632353
GCGTGCC557.415265E-637.6226318
GGGTAAA1850.037.3512271
CGCTGCG1300.037.16924357
CAATGCG7850.036.0250719
ACAGCGT1250.035.8669144
TGCGTAC1350.035.79260360
TAGACAG1650.035.5384185
CGACGAG7800.035.37170824
ATCGTCA8000.034.961310
TCGCTGC1400.034.5094956
CAGCGTT1300.034.50757245
Produced by FastQC (version 0.11.5)