FastQCFastQC Report
Thu 26 May 2016
ERR1356917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764180
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23840.3119683844120495No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17990.23541573974717997No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG14850.1943259441492842No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12660.16566777460807663No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT9140.11960532858750557No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9060.11855845481431077No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG9050.11842759559266142No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA8660.11332408594833678No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA8320.1088748724122589No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG7920.1036405035462849No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC7680.10049988222670052No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT200.007367319451.733638
GCATATA1300.045.15431
CTCGGTG1000.041.3868836
TAACCAC1750.039.4496546
ATGCTTA451.0015742E-438.33122316
GTGGTCG1009.094947E-1237.9479140
CAATGCG3750.037.7179219
AGTACTC753.9895895E-836.829335
ATCGTCA3850.035.8445310
GATCTCG1350.035.77112233
GAGTCCT3800.035.40362528
CGTATCA1201.8189894E-1234.498148
GGTCGCC1201.8189894E-1234.498142
CGACGAG3900.034.49584224
TCTCGGT1300.034.48906735
GTCATCC4200.033.6789213
GTATATG1750.033.5431981
CCGTATC1253.6379788E-1233.1160147
GGGTAAA851.198423E-732.4985961
CCACATT2050.031.973859
Produced by FastQC (version 0.11.5)