FastQCFastQC Report
Thu 26 May 2016
ERR1356917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764180
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA44300.5797063519066188RNA PCR Primer, Index 17 (96% over 28bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25030.32754063178832216No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17750.2322751184275956No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG15860.2075427255358685No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12060.15781622130911566No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA11600.15179669711324556RNA PCR Primer, Index 17 (96% over 28bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9230.12078306158234971No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG9130.11947446936585622No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA9000.11777329948441466No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA8740.11437095972153158No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT8640.11306236750503806No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG7970.10429479965453166No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC7940.1039022219895836No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7700.10076160066999922No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAA252.5716942E-455.17801716
ACCGAGA200.00735635451.75309465
GGGGCCG350.001349741839.41544714
CGACGAG2800.039.4128724
CCGAAAC350.00135017639.4128718
CGATCTC350.00135017639.4128724
CAATGCG2850.038.72141319
GAGTCCT3050.036.18230428
ATCGTCA3100.035.65704710
TCCCCCG400.00259003634.51561760
CCGTATT905.2059477E-934.4862627
GATCTCA400.00260086834.4862625
CCCCGTA958.822099E-932.67119625
TCAAGAC1600.032.3351023
GGGTAAA751.6555878E-632.2314641
GTGTTCA652.3009386E-531.8856289
GTCAGCG3300.031.35114343
TGGTCGT3200.031.2715951
CGTATTA1001.454282E-831.03763628
GTCGTCT3450.030.98765453
Produced by FastQC (version 0.11.5)