FastQCFastQC Report
Thu 26 May 2016
ERR1356916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094613
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG4400.031.36124819
GCTCGAT4850.028.4514464
TGCGACG4700.027.89021922
GTACTAG5750.027.6180951
ATCGTCA4650.027.44826710
TGGTCGT5150.026.80142651
CTCGATA5200.026.54129265
TCGTCTA5000.026.22519754
CGATAAT5400.026.19723967
TCGATAA5300.026.04051466
GCTCGTC5450.025.94622239
TACGTGT5400.025.5582869
GTCGTCT5350.025.15451453
CGTCATT5500.025.08899526
CGACGAG4700.024.95554524
ATTAGGT6100.023.75228919
GCGCTGG6550.023.17168847
ATGCGAC6450.022.99719821
CTGGTCG6200.022.8169550
AGTCCTA5200.022.55803529
Produced by FastQC (version 0.11.5)