FastQCFastQC Report
Thu 26 May 2016
ERR1356916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094613
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGT4600.033.7571151
CTCGATA4500.033.73581365
TCGATAA4550.033.3650966
GCTCGAT4550.033.3650964
CGACGAG3400.032.45962524
TCGTCTA4700.032.30467654
CATTGCG5200.031.83540329
GCTCGTC5100.031.78338439
GTCGTCT4800.031.6143253
CGATATA553.2494208E-431.37597862
CGTCATT5300.031.23473226
CAATGCG3800.030.85799819
ATCGTCA3900.030.1025210
CGATAAT4950.029.96778967
CTGGTCG5000.029.6762550
GTCGTCA5800.028.54208424
CGTCTAC5800.027.96278455
ATTAGGT6000.027.59068119
TGCGACG4300.027.2698622
GTCTACC5700.027.24257956
Produced by FastQC (version 0.11.5)