ERR1356914_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1356914_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1616774
Sequences flagged as poor quality	0
Sequence length	75
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	4849	0.2999182322328291	RNA PCR Primer, Index 46 (96% over 28bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG	4418	0.2732602082913258	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	3810	0.2356544575803421	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	2815	0.17411215172930786	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC	2642	0.16341183121450492	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG	2503	0.15481446386446096	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA	2208	0.13656825258199354	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA	1987	0.1228990570110603	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA	1857	0.11485835373404076	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	1857	0.11485835373404076	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT	1753	0.1084257911124251	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG	1738	0.1074980176573844	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG	1650	0.10205508005447884	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCCG	55	2.6029738E-9	50.171875	5
TTACCGG	30	6.3232036E-4	45.983776	42
TACCGGA	30	6.3232036E-4	45.983776	43
GTGCATT	40	4.9905262E-5	43.143147	61
CCCGGCG	65	1.1312295E-8	42.49387	8
CGCGGCG	35	0.0013503203	39.41466	36
CGTAGCG	35	0.0013503203	39.41466	36
CAATGCG	690	0.0	37.986595	19
CGACGAG	680	0.0	37.0237	24
ATCGTCA	690	0.0	36.527794	10
GAGTCCT	710	0.0	35.94506	28
TGGTCGT	1065	0.0	35.64107	51
ATGTGCG	50	1.8534438E-4	34.52627	11
TCAAGAC	170	0.0	34.493164	3
CGCAGCG	110	2.7284841E-11	34.489964	14
CGGATTC	40	0.002601142	34.48783	38
TGCGATG	60	1.3461335E-5	34.48783	21
ATCGCCG	110	2.910383E-11	34.48783	39
GTCAGCG	1180	0.0	33.61102	43
GTCTACC	1195	0.0	33.21275	56