FastQCFastQC Report
Thu 26 May 2016
ERR1356912_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356912_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850450
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC13910.163560467987536No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGC12370.14545240754894467No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAG11390.13392909636075018No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAG11300.13287083308836498No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTT11060.13004879769533778No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGCGTTCTATCATTCTAGCCTCG9810.11535069668998765No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAAC9050.10641425127873479No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCCCAGGAAATGTTGAGGGAAGAATGTT8550.10053501087659475No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT306.3114497E-445.99862710
ACCCTTG4150.033.2481087
GCTCGAT1900.030.867564
GTAAGCG450.004621364630.66755719
TAAGCGT450.004621364630.66755720
AACCCTT4450.030.2439526
ATCGTCA1950.030.07602710
CTCGATA2000.029.32757465
GTAAGGT953.1865238E-729.0688694
GTGTAGC605.403329E-428.7660681
ACGAACT500.007730489627.6024265
CGACAGC500.00774604227.59105959
GTATTGG5150.027.4813691
CAATGCG2050.026.9276119
AATCTAC658.65079E-426.54079465
TGCGACG2100.026.28183722
CTTGGCC5150.026.12543310
TTGGCCT5000.025.52923811
TGGTCGT2300.025.50373851
CCCTTGG5700.025.4173038
Produced by FastQC (version 0.11.5)