FastQCFastQC Report
Thu 26 May 2016
ERR1356912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850450
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC14670.17249691339878886No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGC12290.14451172908460228No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAG11590.13628079252160621No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTT11270.1325180786642366No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAG11120.13075430654359457No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGCGTTCTATCATTCTAGCCTCG9040.106296666470692No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAAC8810.10359221588570756No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCCCAGGAAATGTTGAGGGAAGAATGTT8630.10147568934093716No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCGC200.00736969451.7298931
CGTTAAC306.264214E-446.0687981
GTCAGCG2150.041.70472343
TGGTCGT2350.039.64620651
CGATTCG350.001350197239.4132533
GCTCGAT2200.039.2100664
GCTCGTC2500.037.24552539
ACTCCCG952.1827873E-1036.34441411
CTCGATA2400.035.94255465
GCGCTGG2500.035.88715747
TCAGCGC2500.035.86606244
GTCGTCT2600.034.48659553
CGCCAGC2800.034.48659534
TCGTCAG2800.033.25493241
GCTTATG2250.032.248161
GTTTAGA553.227904E-431.4105431
GTATACT553.2558813E-431.3643574
CGCTGTA1101.0622898E-931.3514516
GATCGGC1001.4420948E-831.06533262
GACCGTC1800.030.65475322
Produced by FastQC (version 0.11.5)