FastQCFastQC Report
Thu 26 May 2016
ERR1356911_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356911_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719341
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAA1452.1827873E-1128.55077466
TAGCGTA500.00772902527.60292457
GTATCAA12350.026.2838361
TCGTCTA1501.038643E-925.306254
GGTATCA7900.024.9158441
AGGCGAG700.0013341624.6403227
GCTCGAT1701.6734703E-1024.35043564
CTCGATA1752.401066E-1023.65635765
CTAGTAC2250.023.0216583
CGATAAT1803.4197E-1022.99923567
CGTCATT2005.2750693E-1122.41645626
ATCAACG13950.022.0314793
GCGCCTA953.0590015E-421.79481553
TATCAAC14500.021.672112
TCAACGC14500.021.672114
CAACGCA14350.021.4128885
GTTATAA1507.25835E-720.7194921
AACGCAG15400.020.3941976
CGACTAT850.004114521720.29767846
CGTACGA850.00412614120.28779860
Produced by FastQC (version 0.11.5)