FastQCFastQC Report
Thu 26 May 2016
ERR1356911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719341
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTA200.007369801451.72888639
CGACGAG1102.7284841E-1134.48592424
CGATAAT751.6688718E-632.1980567
CAATGCG1305.456968E-1231.8331619
ATGCGAC1551.8189894E-1228.92367721
CCCGTTT1103.646528E-828.23734963
TGCGACG1353.074092E-1028.09964222
TCGATAA1101.1495922E-625.09637566
GTCGACG1101.1558786E-625.08067336
CGCACTA1551.5133992E-924.49601758
TCGACGA1151.7022085E-623.99020837
CGACGAA1202.464436E-622.99061838
ATCGTCA1704.3746695E-922.34859810
GGTATCA10000.022.1186541
GTATATA1906.5847416E-1021.827621
GTACTAG1605.6061253E-821.6002481
TGGTCGT1605.7061698E-821.56419451
GCTCGAT1304.9083174E-621.23539264
TGATCGC850.004092023220.3169089
AATGCGA2051.7462298E-920.18688420
Produced by FastQC (version 0.11.5)