FastQCFastQC Report
Thu 26 May 2016
ERR1356910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468548
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTT200.007353350551.75528752
GGGTAAA501.8493022E-434.5330431
TGTATAC605.387081E-428.7775363
GACCGTC500.007738552527.59397522
GTCCTAC1705.456968E-1226.4076211
GCTCGAT1457.021299E-1026.16949564
TATATAC809.4879935E-525.8997843
ACCGTCG700.00133436324.63747823
TCGTCTA1701.6734703E-1024.35542954
GTGATCG851.4436535E-424.3476268
CAATGCG1151.6925569E-624.00245319
GTATAGT750.001980211423.0220284
GCGTGCT750.001992559522.99743713
TGCGACG1052.2808903E-522.9949822
CGACGAG1103.267081E-521.9497524
TGATCGC953.066389E-421.7847169
CCGTCGG800.002900565321.55779324
GTCTACC1959.276846E-1021.23293956
CTGTGCG2600.021.2261339
CGTCTAC2001.2714736E-920.70211455
Produced by FastQC (version 0.11.5)